Crop Genebank Knowledge Base

Cassava manual

Cassava in vitro processing and gene banking

Download this manual in pdf format here (0.8MB)

This manual describes the cassava in vitro gene banking process set up by IITA.

Within the last 3 years, this standardized process has been successfully used to duplicate over 2000 accessions of cassava from field to in vitro culture. The manual also describes minimum requirements in terms of equipment and consumables for cassava in vitro propagation and gene banking.

Compilation by: Dominique Dumet, Abigael Adeyemi, and Omena Ojuederie

Intended use:

Best practice reference manual for genebank staff.
Provide the frame for further development of ISO normalization (quality control of in vitro germplasm) for cassava gene banking.
Support capacity building in in vitro culture and gene banking.

This manual is accessible on IITA website and available in hard copy (upon request).

Intended users: Genebank managers, supervisors, and staff and trainees

Contents

Introduction

A Explant production
B Germplasm in vitro introduction
C Germplasm in vitro multiplication
D Germplasm gene banking

D1 Transfer to genebank
D2 Germplasm regeneration
D3 Germplasm monitoring
D4 Germplasm inventory

E Germplasm acclimatization
F Basic equipment/items required for cassava gene banking
G Media and stock solutions preparation and storage
H General recommendations for laminar flow room, growth room and genebank maintenance and use