Crop Genebank Knowledge Base

Storage

Storage of forage legume genetic resources in seed banks

Contributors to this page: ILRI, Ethiopia (Jean Hanson); ICARDA, Syria (Ahmed Amri, Kenneth Street, Ali Shehadeh, Natalya Rukhkyan); GRCTPL, Australia (Richard Snowball); Bioversity International/ILRI, Addis Ababa, Ethiopia (Alexandra Jorge).

Contents:
Base collection
Active collection
Storage management

Base collection

When it should be used

All original samples should be placed in long-term storage (original samples are the best representative sample of that accession and storage conditions should be used to retain viability as long as possible).
In addition, representative samples of all accessions should be stored in base collections for long-term storage.

Sample specifications

Minimum sample size/viability for storage

4000 seeds or 5 g for small seeds of 90% germination or 80% germination for wild species (see FAO/IPGRI, 1994).

Moisture content:

3-7% (according to FAO/IPGRI, 1994).

Container specifications

Seed packaging method

Laminated re-sealable aluminium foil packets are moisture proof and very practical, making good use of space.
Use of vacuum for packing is optional.
Packing is best carried out in an air-conditioned room with controlled humidity as soon as possible after drying (rapid packing in a dry environment prevents reabsorption of moisture by the seeds during packing).

Specifications of packaging material

Minimum quality: outer polyester of 12 µm thickness, middle aluminium layer of 9 µm thickness and inner polythene layer of 55 µm (this thickness is impermeable and sufficiently flexible for handling and strong enough for forage legume seeds, which are usually smooth).

Storage specifications

The two commonly available options for seed storage are walk-in cold stores and freezers. The choice depends on the number of accessions to be stored, seed size and storage temperatures selected.

When collections are small and sub-zero temperatures are required, chest or upright freezers are a cheaper option for seed storage.

Assigning location codes

Location codes should specify the exact place in the store (e.g. unit/block number, row number, shelf number and tray/box number) or freezer (e.g. freezer shelf and box number). The exact locations are very important to locate material in the store.
Moveable shelves on tracks are preferred (moveable shelves make best use of space).

Storage conditions

At -18^oC to -20^oC (FAO/IPGRI, 1994).

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Active collection

When it should be used

To store seeds for distribution and use (seed quantities for distribution are usually larger and are not usually stored for long periods, therefore storage conditions are less stringent).

Sample specifications

Minimum sample size/viability for storage

A minimum of 1500 seeds and up to 500 g and above 80% of original germination (sufficient seeds for distribution needs during the period of high viability).

Moisture content

3-7% (according to FAO/IPGRI, 1994).

Container specifications

Seed packaging method

Laminated re-sealable aluminium foil packets are moisture proof and very practical, making good use of space.

or

Plastic or metal containers if store is dehumidified (store must be dehumidified to avoid absorption of moisture from the air surrounding the seeds).

Specifications of packaging material

Laminated aluminium foil: Minimum quality: outer polyester of 12 µm thickness, middle aluminium layer of 9 µm thickness and inner polythene layer of 55 µm (this thickness is impermeable, sufficiently flexible for handling and strong enough for forage legume seeds, which are usually smooth).
Plastic or metal containers: Square-based bottles with a screw cap and inner rubber seal made of pure plastic materials (high quality), semi transparent, white in colour, adequate volume or metal cans of similar design.

Storage specifications

Assigning location codes

Location codes should specify the exact location in the store, e.g. unit/block number, row number, shelf number and tray/box number (exact locations are important to locate and access material in the store).
Moveable shelves on tracks are preferred (moveable shelves make best use of space).

Storage conditions

Between 0 to 10^oC (conditions to maintain seed viability for at least 25 years).

For information on safety duplication, click here.

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Storage management

Storage space arrangement

Moveable racks (this maximizes space between and on the racks in the store and is the most economic way to utilize the space).
Shelves should be spaced slightly more than the height of the containers.

System for tracking material/inventory system

Database of stock and location and use of barcodes (databases are needed to keep track of information. Barcodes help avoid errors in recording).

Recording information during conservation

The following information must be recorded for each accession:

Accession number (ID number).
Lot number (ID number).
Weight of seeds (weight of seeds in store).
Number of seeds (number of seeds in store).
Thousand or hundred seed weight (weight of 1000 or 100 seeds).
Number of packets (number of containers for seeds of one lot of one accession).
Location of containers (store, shelf or box number).
Type of container (material and size of container).
Year of production of seeds (indicates age of seeds).
Flag for regeneration [Y/N].
Flag indicating seed availability and distribution status [A, L, S].
Year of safety duplication (year).
Institute holding the duplicate (name of institute holding the safety duplicate).
Location of duplicate sample (box label where the duplicate sample is placed).

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References and further reading

Bass LN. 1984. Storage of seeds of tropical legumes. Seed Science and Technology 12:395-402.

Cabrales R, Bernal J. 1983. Effect of different systems of seed treatment, packing, and storage on vigor and germination of five tropical forage legumes. XIVth International Grasslands Congress. pp. 263-265.

Chin HF, Hanson J. 1999. Seed storage. In: Loch DS, Ferguson J, editors. Forage Seed Production Vol II Tropical and Subtropical Species. CABI Publishing, UK. pp. 303-315.

FAO/IPGRI. 1994. Genebank standards. Food and Agriculture Organization of the United Nations, Rome and International Plant Genetic Resources Institute, Rome. Available in English, Spanish, French and Arabic.

Keya NCO, van Eijnatten CLM. 1975. Studies on oversowing of natural grasslands in Kenya 1. The effects of seed threshing, scarification and storage on the germination of Desmodium uncinatum (Jacq.) DC. East African Agriculture and Forestry Journal 40:261-263.

Penteado MI de O, Sáenz de Mira LE, Pérez de la Vega M. 1996. Genetic resources of Centrosema spp.: genetic changes associated to the handling of an active collection. Genetic Resources and Crop Evolution 43: 85 90.