Crop Genebank Knowledge Base

The labeling of probes or primers with radioactive tags in order to enable detection of variation in DNA fragments.

(RAPD) A molecular marker technique for amplifying anonymous stretches of DNA using PCR with arbitrary primers.

A sample drawn at random from a larger group.

In studies of hybrid origins of species, investigators may attempt to ???re-synthesize??? a hybrid by crossing its putative parents and compare the natural to the putative hybrid by morphological or molecular markers.

Seeds that loose viability when dried or stored at low temperatures.

The production of a DNA molecule with segments derived from more than one parental DNA molecule. In eukaryotes

Refers to identical or near identical items. In germplasm analyses it refers to (near) duplicate germplasm accessions.

The process of restoring a whole plant from individual cells by manipulating an in vitro culture. The growing of a sample of seeds from an accession to replenish the viability of the original accession. It is usually done when the viability of the original material drops to less than 85%.

The percentage seed viability at or below which the accession must be regenerated to produce fresh seeds.

(RH) A measure of the amount of water present in the air compared to the greatest amount possible for the air to hold at a given temperature, expressed as a percentage. It differs from "absolute humidity", which is the amount of water vapour present in a unit volume of air, usually expressed in kilograms per cubic meter.