Collecting missions

1. Introduction to collecting (0.3 MB)

2. Planning collecting missions (0.3 MB) 

The module on Collecting missions covers four topics:

• reasons for collecting (eg. rescue, immediate use, gap filling, research, etc.)
• types of collecting missions (eg. multi-species, species specific, wild species, crop, single/multiple visits, centralized/decentralized visits)
• dangers of collecting
• ingredients for successful mission.

Ecogeographic Surveys

Ecogeographic Survey course

This module discusses Ecogeographic studies against the background of global genetic erosion. The use of ecogeographic data in conservation activities is emphasized with an example given of planning collecting missions.

You can find examples of ecogeographic surveys and their application in plant genetic resources including:

• the three phases of an ecogeographic survey
• lists the types of geographical, ecological and taxonomic data which are collected in an ecogeographic survey
• identifies problems commonly encountered when recording and analysing ecogeographic data
• reviews the different statistical and graphical methods available for analyzing and displaying ecogeographic data
• lists the contents of an ecogeographic conspectus and report
• summaries the criteria used in setting conservation priorities.

The module includes lecture support notes, slides (PDF), assessment questions and references.

Plant Genetic Resources and Genebank Management

Plant Genetic Resources and Genebank Management Training Module (3.6MB)

This module was developed by Bioversity International with support from Republic of Korea’s Rural Development Administration (RDA) for the International Training Course on Plant Genetic Resources and Genebank Management held in Suwon, Republic of Korea on 7-18 September 2009.

The training course is a joint collaboration of the Bioversity International and RDA with the purpose of contributing to capacity development on genebank management in the Asia Pacific and Oceania region. It aims to train genebank curators and genetic resource professionals on an array of good practices and new developments for effective ex situ conservation and sustainable utilization of genetic diversity.

The course brings together the best in the field to support the implementation of this module. The corps of trainers is composed of experts from RDA, Bioversity International, International Rice Research Institute (IRRI), Asian Vegetable Research Development Center (AVRDC), Food and Agriculture Organization (FAO) and the University of Tsukuba, Japan. Bioversity and RDA acknowledge the support of these international lecturers and their respective organizations.

Bioversity International hopes that this module will not only be useful for this training course but also for implementing what our trainees learned afterwards. Bioversity International encourages trainees to echo what they have learned to their colleagues.

Objectives of this training module:

The course aims to train genebank curators and genetic resource professionals in good practices for effective ex situ conservation and sustainable utilization of genetic diversity held in genebanks.

More specific objectives include:

• To enhance the use of standard protocols for routine genebank operations including how to develop a genebank operation manual;

• To develop a practical understanding of the utilization of genetic resources and the interface between genebanks and breeders;

• To equip participants with essential knowledge in documentation and database management;

• To acquaint the participants with morphological andmolecular techniques to characterize and evaluate plant genetic resources; and

• To enhance awareness of international and national policy instruments and regimes concerning access to and exchange of genetic resources.

Contents:

PART 1. Discussion and Lectures

DISCUSSION 1. Policy Instruments and Regimes for Access and Exchange of Genetic Resources

LECTURE 1. Seed Processing and Storage

LECTURE 2. Germplasm Regeneration

LECTURE 3. Characterization and Evaluation

LECTURE 4. Documentation and Information Management

LECTURE 5. Germplasm Exploration and Collection

LECTURE 6. Use of Molecular Markers for Diversity Analysis

LECTURE 7. Utilization of Plant Genetic Resources in Pre-breeding/Genetic Enhancement

LECTURE 8. Genebank Development and Management

PART 2. Practicum

PRACTICUM 1. Seed Processing and Drying

PRACTICUM 2. Purity and Health Testing

PRACTICUM 3. Storage Conditions, Viability Testing and Monitoring

PRACTICUM 4. Documentation and Information Management

PRACTICUM 5. Practicum 5. Regeneration Plan, Distribution Standards and Collection Security

PRACTICUM 6. Germplasm Exploration and Collection

PRACTICUM 7. Characterization and Evaluation

Citation: Bioversity International and Rural Development Administration. 2009. A Training Module for the International Course on Plant Genetic Resources and Genebank Management. Bioversity International, Rome, Italy.

Module Prepared by: Prof Nestor C Altoveros and Prof Teresita H Borromeo of the University of the Philippines, Los Baños, Laguna, Philippines.

Photo Credits: From left to right. a) Local varieties of rice on display at the CRRA Research Station in Sotuba, Bamako, Mali (P.Bordoni/Bioversity International); b) Long purple aubergines on sale in the Turgetreis Market, Turkey (A.Jarvis/Bioversity International); c) Barley (Bioversity slideshow); d) Background photo: Chillies, aubergines and tomatoes on sale in the Turgetreis Market,Turkey (A.Jarvis/Bioversity International).

Cover Design and Layout: Katrina Borromeo

Molecular Marker Learning Modules: Volumes 1 and 2

These modules aim to promote capacity building and research biodiversity use and conservation worldwide through the application of molecular markers.

They are especially directed to those countries with limited access to up-to-date scientific literature and research technologies. They discuss the fundamental principles of genetic diversity, the qualities of the markers used to measure it and the most widely used technologies, including those based on proteins, DNA and the polymerase chain reaction.

The modules are intended for scientists with a minimal background in genetics and plant molecular biology, but with a working knowledge of plant genetic resources and issues concerning their conservation and management.

Volume 1: Using Molecular Marker Technology in Studies on Plant Genetic Diversity: Learning module M. Carmen de Vicente (IPGRI) and Theresa Fulton (Institute for Genomic Diversity, Cornell University)

Title page (PDF 115 KB)
Preface (PDF 9 KB)
Foreword (PDF 11 KB)
About this module (PDF 13 KB)
Objectives (PDF 7 KB)

I. Introduction (PDF 486 KB)

II. Protein-based technologies
Protein basics (PDF 137 KB)
Isozymes (PDF 295 KB)

III. DNA-based technologies
DNA basics (PDF 394 KB)
Restriction fragment length polymorphisms (RFLPs) (PDF 503 KB)
PCR basics (PDF 326 KB)
PCR with arbitrary primers (PDF 206 KB)
Amplified fragment length polymorphisms (AFLPs) (PDF 279 KB)
Sequence-tagged sites (Microsatellites etc) (PDF 308 KB)
Latest strategies (PDF 324 KB)

IV. Complementary technologies (PDF 136 KB)
Denaturing gradient gel electrophoresis (DGGE)
Thermal gradient gel electrophoresis (TGGE)
Single-stranded conformational polymorphism (SSCP)
Heteroduplex analysis
Denaturing high-performance liquid chromatography (DHPLC)

V. Final considerations (PDF 152 KB)
Considerations when choosing a technique
Practical applications

Glossary (PDF 33 KB)
Feedback form (PDF 14 KB)
Acknowledgements (PDF 11 KB)
Copyright (PDF 50 KB)

Volume 2: Genetic Diversity Analysis with Molecular Marker Data: Learning Module M.C. de Vicente (IPGRI), C. Lopez (Universidad Nacional Agraria 'La Molina', Peru) and T. Fulton (Institute for Genomic Diversity, Cornell University)

Title page (PDF 80 KB)
Foreword (PDF 27 KB)
About this module (PDF 75 KB)
Objectives (PDF 60 KB)

I. Introduction (PDF 519 KB)

II. Basic concepts of population genetics (PDF 630 KB)

III. Measures of genetic diversity (PDF 342 KB)

IV. Software programs for analysing genetic diversity (PDF193 KB)

V. Glossary (PDF 80 KB)

VI. Feedback form (PDF 47 KB)

VII. Acknowledgements (PDF 30 KB)

VIII. Copyright (PDF 71 KB)

IX. Appendixes

Appendix 1: Basic Concepts of Population Genetics: Critical values of the chi-square distribution (PDF 37 KB)

Measures of Genetic Diversity
Appendix 2: Analysis of molecular variance: Example 1 (PDF 40 KB)
Appendix 3: Analysis of molecular variance: Example 2 (PDF 40 KB)
Appendix 4: Geometric distance (PDF 50 KB)
Appendix 5: Transforming data from quantitative variables: An example (PDF 53 KB)
Appendix 6: Applying the simple matching coefficient for morphological characters (categorical variables) (PDF 54 KB)
Appendix 7: Calculating Nei’s genetic distance (PDF 58 KB)
Appendix 8: Morphological and molecular similarities (PDF 176 KB)
Appendix 9: Software Programs for Analysing Genetic Diversity (PDF 31 KB)