CGKB News and events Procedures
Please click on the boxes in the diagram below or use the menu on the left to go to the topic of your interest.
List of equipment and supplies for genebanks
|
Print/PDF version (0.09 MB) |
.jpg)
Page compiled by: Bioversity International/ILRI, Addis Ababa, Ethiopia (Alexandra Jorge); ILRI, Addis Ababa, Ethiopia (Jean Hanson) including information extracted from: Rao NK, Hanson J, Dulloo ME, Ghosh K, Nowel D and Larinde M. 2006. Manual of seed handling in genebanks. Handbooks for Genebanks No. 8. Bioversity International, Rome, Italy. 147pp.
(The list is not exhaustive and mention of suppliers does not necessarily constitute endorsement)
|
No. |
Item |
Specification/Purpose |
Supplier |
|
1 |
Balance, analytical |
Weighing up to four decimal places, required for seed moisture content determination using small samples |
Mettler-Toledo (Schweiz) AG
|
|
2 |
Balance, moisture determination |
Combining heating with highly accurate weighing technology to deliver a fast and precise method of |
Mettler-Toledo (Schweiz) AG
|
|
3 |
Cold rooms |
Cold storage modules made of prefabricated polyurethrane insulating panels; panels should be 150 mm thick and constructed for long-term storage (-20°C) or 75 mm thick and for medium-term storage (+5°C); floor insulated and heavy duty, door-hinged type; heated seal for long-term storage, fitted with transparent PVC strip curtain; refrigeration system to provide adjustable controlled environment of
|
BMIL International, Inc. |
|
4 |
Containers
b. Aluminium cans; Glass bottles |
Aluminium foil bags - laminated, made of three layers: polyester on the outside, aluminium in the middle and polyethylene on the inside, and puncture resistant
Cans, sealed or with screw caps fitted with rubber gasket; Glass bottles fitted with polypropylene lids
|
Barrier Foil Products Co.
|
|
5 |
Data loggers |
Continuous logging of temperature and RH data in cold rooms and freezers and to record field observations.
|
OAKTON Instruments
|
|
6 |
Dehumidifier |
Rotary type with high performance activated silica gel or other desiccant wheel |
Bry-Air Inc.
Munters Limited
|
|
7 |
Distillation apparatus |
Distilled water for germination testing etc.
|
Cole-Parmer Instruments Co.
Fisher Scientific
Osaw Industrial Products Pvt. Ltd.
Thomas Scientific
|
|
8 |
Drying cabinet/room |
Rotary absorption dehumidifiers with secondary refrigeration equipment to provide an environment of 15°-20°C and 15-20% RH for seed drying |
Bry-Air Inc.
Hoffman Manufacturing Co.
Huurre Group Oy. PO Box 127
Munters Limited
Watford Refrigeration & Air Conditioning Ltd.
|
|
9 |
Electronic scale |
Weighing up to two decimal places – required at various stages of the seed-handling
|
Hoffman Manufacturing Co.
Seedburo Equipment Co.
Cole-Parmer Instruments Co.
Fisher Scientific
Osaw Industrial Products Pvt. Ltd.
Thomas Scientific
|
|
10 |
Freezer (chest type/ vertical) |
Standard domestic freezers providing -20°C for long-term seed conservation.
|
Locally available (e.g. Revco, Kelvinator, Westinghouse and others)
|
|
11 |
Germination cabinet |
Providing very high RH levels, illuminated, with diurnal-cycling control allowing independent selection of day/night temperatures and light
|
Controlled Environments Limited
Hoffman Manufacturing Co.
Seedburo Equipment Co.
Weiss Gallenkemp Ltd.
|
|
12 |
Global positioning System (GPS) |
Portable and handheld to carry on collecting trips
|
Garmin International Inc.
|
|
13 |
Grinder (coffee mill) |
Grinding small quantities of seeds for moisture determination
|
Locally available (Braun, Moulinex, etc)
|
|
14 |
Incubator |
With diurnal-cycling control allowing independent selection of day/night
|
Percival Scientific, Inc
Weiss Gallenkemp Ltd
Cole-Parmer Instruments Co.
Fisher Scientific
Thomas Scientific
|
|
15 |
Magnifier lamp |
Seed cleaning
|
Hoffman Manufacturing Co.
Seedburo Equipment Co.
|
|
16 |
Moisture testers a. Quick meter
b. Hygropalm |
Quick assessment of moisture content
Non-destructive moisture testing
|
Hoffman Manufacturing Co.
Seedburo Equipment Co.
Rotronic Instruments (UK) Ltd
|
|
17 |
Oven (mechanical/ |
Temperature range 30°–200°C, with fan convection and temperature controller
|
Cole-Parmer Instruments Co.
Fisher Scientific
Osaw Industrial Products Pvt. Ltd.
Thomas Scientific
|
|
18 |
Purity workboard |
Seed cleaning
|
Hoffman Manufacturing Co.
Seedburo Equipment Co.
Osaw Industrial Products Pvt. Ltd.
|
|
19 |
Sealing machine |
Constant heat machines that use thermostatic controllers to maintain the
|
Aluminium foil bags:
Hulme-Martin Tavak
Can sealer:
|
|
20 |
Seed blower |
Seed cleaning – separation of lightweight material from seeds
|
Hoffman Manufacturing Co.
Seedburo Equipment Co.
Osaw Industrial Products Pvt. Ltd.
|
|
21 |
Seed counter |
Counting a predetermined number of seeds or registering a count on a
|
Hoffman Manufacturing Co.
Seedburo Equipment Co.
Osaw Industrial Products Pvt. Ltd.
|
|
22 |
Seed-counting boards |
For counting and spacing large seeds in planting medium
|
Hoffman Manufacturing Co.
|
|
23 |
Seed divider |
To prepare representative samples from composite samples
|
Hoffman Manufacturing Co.
Seedburo Equipment Co
Osaw Industrial Products Pvt. Ltd.
|
|
24 |
Shelving (mobile/ |
Mobile galvanized steel frame with PVC coating similar to library shelves preferable
|
Crown Industrial
Montel
|
|
25 |
Sieves, graded |
Seed cleaning and separation
|
Hoffman Manufacturing Co.
Seedburo Equipment Co
Osaw Industrial Products Pvt. Ltd.
|
|
26 |
Stereo microscope |
Seed quality and health assessment
|
Cole-Parmer Instruments Co.
Fisher Scientific
Osaw Industrial Products Pvt. Ltd.
Thomas Scientific
|
|
27 |
Storage trays (metal/ |
Should withstand below-freezing
|
Local suppliers
|
|
28 |
Supplies, General a. Germination paper
b. Labware (petrishes,
c. Field (seed
|
|
Hoffman Manufacturing Co.
Seedburo Equipment Co.
Whatman plc.
Cole-Parmer Instruments Co.
Fisher Scientific
Osaw Industrial Products Pvt. Ltd.
Thomas Scientific
A.P. Burt & Sons
PBS International
M/S Ajay Kumar & Co.
Sigma-Aldrich
Merck Chemicals Ltd.
|
|
29 |
Thermohygrometer, |
To monitor temperature and RH in cold rooms
|
Cole-Parmer Instruments Co.
Fisher Scientific
Thomas Scientific
|
|
30 |
Thresher, mechanical |
Designed for threshing single plants and heads of small cereal grains
|
Hoffman Manufacturing Co.
Seedburo Equipment Co.
|
Subcategories
-
main
- Article Count:
- 1
-
Collecting
- Article Count:
- 31
-
Acquisition/Registration
- Article Count:
- 2
-
Sample processing
- Article Count:
- 1
-
Quality testing
What is quality testing?
The quality testing of seeds or plant materials assures that the materials to be conserved are in good conditions, i.e. can be grown again (viable) and are free of external contaminants (pests and diseases) and external genes (artificially produced genes). They are composed by three major aspects:
- Viability testing
- Plant health
- Transgenes.JPG)
The quality of seed can be tested with a germination test
- Article Count:
- 5
-
Methods of conservation
- Article Count:
- 2
-
Cold storage
- Article Count:
- 1
-
Tissue culture
- Article Count:
- 1
-
Cryopreservation
- Article Count:
- 1
-
Molecular
- Article Count:
- 1
-
In field conservation
- Article Count:
- 1
-
Characterization
- Article Count:
- 1
-
Regeneration
What is Regeneration?
Regeneration is the renewal of germplasm accessions by sowing seeds or planting vegetative materials and harvesting the seeds or plant materials which will posses the same characteristics as the original population.
Germplasm regeneration is the most critical operation in genebank management, because it involves risks to the genetic integrity of germplasm accessions due to selection pressures, out-crossing, mechanical mixtures and other factors. The risk of genetic integrity loss is usually high when regenerating genetically heterogeneous germplasm accessions. Germplasm regeneration is also very expensive..JPG)
Regeneration on fields
Why should germplasm be regenerated?
Germplasm is regenerated for the following purposes:
1. To increase the initial seeds or plant materials
In new collections or materials received as donations, the quantity of seeds or plant materials received by the genebank is often insufficient for direct conservation. Seeds or plant materials may also be of poor quality due to low viability or infection. All these materials require regeneration. Newly acquired germplasm of foreign origin may need to be initially regenerated under containment or in an isolation area under the supervision of the national phytosanitary authorities.
2. To replenishing seed stocks or plant materials in active and base collections
Increase seed stocks or plant materials of accessions that have:
- Low viability identified during periodic monitoring;
- Insufficient stocks for distribution or conservation.
Active collections should be regenerated from original seeds or plant materials in a base collection; this is particularly important for out-breeding species. Using seeds from an active collection for up to three regeneration cycles before returning to the original seeds or plant materials (base collection) is also acceptable (FAO/IPGRI 1994).
Base collections should normally be regenerated using the residual seed or plant materials from the same sample.How is it done?
If possible, regenerate germplasm in the ecological region of its origin. Alternatively, seek an environment that does not select some genotypes in preference to others in a population.
If no suitable site is found, seek collaboration with an institute that can provide a suitable site or regenerate in a controlled environment such as a growth room.
Examine the biotic environment in the context of prior information about the plants and past experience - an inappropriate biotic environment can be detrimental to plants, seed or propagation materials quality and the genetic integrity of an accession.Meeting special requirements
There may be special requirements for regeneration of accessions with special traits that breeders and researchers use frequently—such as high-yielding, pest-and disease-resistant accessions and genetic stocks — or if there are insufficient seeds for safety duplication and repatriation.
The following factors when regenerating germplasm accessions must be consider:- Suitability of environment to minimize natural selection;
- Special requirements, if any, to break dormancy and stimulate germination (such as scarification);
- Correct spacing for optimum seed set; and
- Breeding system of the plant and need for controlled pollination or isolation..JPG)
Regeneration in a protected environment
When should it be done?
It should be done when either the quantity and/or the quality of a particular seed or plant material are not sufficient in a genebank.
The regeneration of accessions that have inadequate quality (low viability) should take priority over that of accessions with inadequate numbers of seeds or planting materials.
The regeneration of accessions in base collections should take priority over regenerating those in active collections.
- Article Count:
- 1
-
Dissemination
- Article Count:
- 1
-
Safety duplication
- Article Count:
- 1
-
Information/Documentation
- Article Count:
- 1
-
List of equipment and supplies
- Article Count:
- 1
